This test uses immunological reactions performed on a test strip by migration. Adenovirus diagnostic testing market growth analysis 2020. Fecal rotaadeno duo antigen rapid test kit abin5186. Rapid diagnosis of rota adenoviruses for acute gastroenteritis in hospitalized children under 4 years old, baghdad razaq hadi eissa alsayidi1.
Rotavirus epidemiology ods in bangui, central african. M dugua 1 introduction results acute gastroenteritis age is one. In the analysis of rotavirus, the molecular and immunological methods had an agreement of 83%, while for adenovirus the agreement. Detection of rotavirus, norovirus and adenovirus by. Objective we conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse. Immunochromatographybased diagnosis of rotavirus infection. Specimens were obtained from 1,350 hospitalized patients and. The vikia rotaadeno test gave a positive result in 24239 10. The samples were analyzed using vikia rotaadeno test kit biometrieux. Biomerieux vikia rota adeno vzporedni test za rotaviruse in adenoviruse iz humaneg fecesa bm31111 kratka navodila za rokovanje z instrumentom. Rotavirus is a virus that leads single cause of severe diarrhea among infants and young children and is.
Rapid communications evidence of falsepositive results in a. Sensibilite et specificite du test immunochromatographique vikia. Vikia rotaadeno is a rapid, qualitative, chromatographic immunoassay for the simultaneous detection of rotavirus and adenovirus. The study was part of a rotavirus surveillance project in niger. We excluded rotavirus specimen tests where age or sex was missing n21. Enteric prevalence of rotavirus and adenovirus in under. M dugua 1 introduction results acute gastroenteritis age is one of the most common diseases in humans and continues to be a cause of high morbidity and mortality in children. Orion diarlex rota microgen rotascreen eia in house pcr biomerieux vikia coris combistrip biokit biorapid novamed rotastick rbiopharm ridaquick rota certest rota adeno card certest rota card atlas medical rota adeno biosigma fast step oxiod prospect quadratech check4 rotavirus rbiopharm ridaquick kombi. Patientswith ibd were treated with sulfasalazine or mesalazine, azathioprine, cyclosporine, steroids and infliximab according to accepted clinical protocols.
Diagnosis of viral gastroenteritis by simultaneous. Diagnosis of viral gastroenteritis by simultaneous detection. Tests for the rapid diagnostic of rotavirus english. In terms of frequency, rotavirus was detected in10 10% of the samples followed by adenovirus type 4041 44%. Loinc 803767 rotavirus and adenovirus ag panel stool.
Comparison of vikia rotaadeno to ideia with 189 stool samples. Feb 17, 2020 the pdf for the study can be requested using the following link. Accurate detection of rotavirus is essential for prevention and control of rotavirus outbreaks and disease monitoring. The multiplex assays were clinically evaluated using 239 fecal samples submitted to our laboratory over a 1year period for the routine detection of rotavirus andor adenovirus antigens using the vikia rota adeno test biomerieux, boxtel, the netherlands. Diagnostic accuracy of seven commercial assays for rapid. We conducted a parallel evaluation of the diagnostic accuracy of vikia rota adeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Vikia rota adeno kit is a qualitative test based on the association of monoclonal antibodies. Vikia rotaadeno is a simple rapid test used as an aid in the immediate diagnosis of viral gastroenteritis by rotaviruses and adenoviruses. Rotavirus a and adenovirus in samples of human faeces were compared. Vikia rota and adeno biomerieux france is a qualitative test based on the immunochromatography technique ict.
The full text of this article is available in pdf format. Edelleen ripulitauteihin kuolee koko maai lmassa vuosittain 2,5 miljoona lasta. Vikia rotaadeno and 10 56 % were positive when analysed by rtpcr table 1. Etiology of febrile illnesses in young children attending. Adeno ja rotaviruksia osoittavien vier i testien vertailu. We therefore reexamined samples initially testing pos itive in the vikia rotaadeno ict with other commercially available elisa rotavirus assays and, for a subset of specimens, by rtpcr. Objective we conducted a parallel evaluation of the diagnostic accuracy of vikia rota adeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse. Europe pmc is an archive of life sciences journal literature. Genotype distribution and phylogenetic analysis of. M dugua 1 introduction results acute gastroenteritis age is one of the most common diseases in humans and. Vikia rotaadeno biomerieux diarlex mb fumouze b the two immunochomatographic tests were performed in the clinical laboratory of the debrousse hospital. Letters rotavirus epidemiology in bangui, central african and vp7 and vp4 were amplified by republic, 20081 to the editor.
Rapid diagnosis of the infectious agent avoids unnecessary antibiotic treatment and leads to rapid isolation of infected patients. Pdf diagnostic accuracy of vikia rotaadeno and premier. Group a rotaviruses rvas are the primary cause of acute gastroenteritis. Infection with group a rotavirus is among the lead. The pathflow rota adeno combi test cassette is a rapid chromatographic immunoassay for the qualitative detection of rotavirus and adenovirus in human faeces, providing results from 10 minutes offering an easytouse, rapid alternative. Rapid test for the qualitative dual detection of rotavirus and adenovirus in human stools. Enteric adenovirus kit pcr positive negative sensitivity % specificity % ppv % npv % vikia positive 8 0 negative 2 190 80 100 100 98.
Enteric prevalence of rotavirus and adenovirus in under five. Vikia rota adeno is a rapid, qualitative, chromatographic immunoassay for the simultaneous detection of rotaviruses and adenoviruses, which are responsible for the majority of pediatric diarrhea cases. Clostridium difficile infection in polish pediatric. All reagents and stool samples were allowed to reach room temperature before use. This term was created for, but is not limited in use to, the biomerieux vikia rota adeno one step combo card test, which is intended for the dual detection of rotaviruses and adenoviruses in a single stool extract. Rotavirus epidemiology ods in bangui, central african and. L, zaragoza, spain and vikia rota adeno biomerieux sa, lyon, france were retrospectively evaluated using realtime pcr as reference test. Biomerieux vikia rotaadeno vzporedni test za rotaviruse. Serodetection of rotavirus and adenovirus gastroenteritis co. The main objective of this study was to identify rotavirus and adenovirus in under five children predisposed with acute diarrhea in yaoundecameroon using vikia rotaadeno rapid test kit biomerieux, france. During a 24month period from 1 may 2010 adults 15 years with age admitted to one of four hospitals in the central denmark region were examined for rotavirus with vikia rota adeno rapid test in addition to routine culture for bacterial pathogens. In the study, one hundred children less than 5 years of age with acute gastroenteritis admitted to sulaimanipaediatric hospital were studied. Herve crehalet, francois vandenesch, anne marie freydiere.
Adenovirus diagnostic testing market growth analysis 2020 by. Diagnostic accuracy of vikia rotaadeno and premier. A total of 250 infants and young children with diarrhea 159 male and 91 female, mean age 8. In our study, the evaluation was done after optimization of the manufacturing process for vikia rotaadeno biomerieux personal communication, which may explain the difference between the australian study and this study. Rotavirus epidemiology in bangui, central african republic. Enteric prevalence of rotavirus and adenovirus in under five children predisposed to acute diarrhea in yaoundecameroon ngum vn. Results obtained by the vikia rotaadeno test revealed that 100 40% of these children were infected with rotavirusa, mostly male children 61100, p vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Many diagnostic laboratories in australia use the vikia rotaadeno assay for detection of rotavirus in faecal specimens. Detection of rotavirus, norovirus and adenovirus by molecular. Pdf sensitivity and specificity of the vikia r rota. Performance analysis of two immunochromatographic assays for. Oct 23, 2017 we conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard.
Adiogo d, fokunang cn, gonsu kh, tembefokunang ea, abena omt. If no stool sample was obtained during the baseline visit, parents were requested to collect a stool sample and bring it into the health care center within four days. Oct 10, 2016 one stool sample was collected for rotavirus testing vikia rotaadeno immunochromatographic test biomerieux from each child sensitivity 96. Children aged 5 years and under 59% samples had a virus 36. Pdf sensitivity and specificity of the vikia r rotaadeno. The incidence of rotavirus and adenovirus infections among.
The multiplex assays were clinically evaluated using 239 fecal samples submitted to our laboratory over a 1year period for the routine detection of rotavirus andor adenovirus antigens using the vikia rotaadeno test biomerieux, boxtel, the netherlands. Rapid communications evidence of falsepositive results in. Acute diarrheal illness was defined as occurrence of. Acute diarrhea in tropical countries can be caused by a broad spectrum of viral, parasitic and bacterial enteropathogens. Pdf objective we conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and. Evaluation of the bionexia rotaadeno rapid test h briand 1, l ji 2, j morgand 1, l thery 3, m bidalot 3, k ambertbalay 3, j kaplon 3 a duraffourgcollomb 1, j. Enteric prevalence of rotavirus and adenovirus in under five children predisposed to acute diarrhea in yaounde. This observation underlines the importance of regular assess. Biomerieux vikia rotaadeno vzporedni test za rotaviruse in. We conducted a parallel evaluation of the diagnostic accuracy of vikia rotaadeno, a rapid diagnostic test rdt and premier rotaclone an enzyme immunoassay eia using reverse transcription polymerase chain reaction rtpcr as the reference standard. Navodila za uporabo instrumenta naj bodo zmeraj v blizini naprave. Diagnostic accuracy of seven commercial assays for rapid detection of group a rotavirus antigens jerome kaplon, aceline fremy, sylvie pillet,b lucile mendes martins,c katia ambertbalay,a serge l. Rotavirus infection in children and adult patients attending in a. Tests for the rapid diagnostic of rotavirus english translation.
Rotavirus infection in children and adult patients. Multicenter prospective study on the burden of rotavirus. If the sample is adeno positive, we recommend to further confirm the serotype result with 4041 adeno strip. Pred uporabo dobro preberite tudi originalna navodila. The elisa test, ideia from dako, considered as the reference method was blindly performed by biomerieux laboratory. Rotavirus was detected every month during the period of the study, with peak of positivity between june and august 35%. Trends of viral gastroenteritis in the australian capital. Oct 23, 2017 europe pmc is an archive of life sciences journal literature. Vikia rotaadeno biomerieux, france streptococcus quickvue inline strep a test quidel, usa, and urine dipsticks urilab h10. Tutkimuksen tarkoituksena oli vertailla ka ytossa olevaa diarlex mb vieritestia rida quick ja vikia vieritesteihin.
The study protocol was approved by the institutional ethical. Rotavirus infection in children and adult patients attending. After receiving concerns regarding this test, company subsequently performed a parallel evaluation of the diagnostic accuracy of vikia rota adeno. Of the 24 hrv positive samples, 19 were confirmed with the multiplex pcr assay and 4 additional positive samples were detected, making a total of 9 discrepant samples. For adenovirus, vikia rotaadeno kit produced 1 positive result 5. After informed consent and epidemiologic and clinical data had been obtained, a fecal specimen was collected from each child. Biomerieux vikia rotaadeno vzporedni test za rotaviruse in adenoviruse iz humaneg fecesa bm31111 kratka navodila za rokovanje z instrumentom. The aim of this study was to determine the incidence and clinical significance of rotavirus and enteric adenovirus gastroenteritis and also to determine possible risk factors for rotavirus and adenovirus gastroenteritis using new simple rapid screening test vikia rota and adeno. Ict assay biomerieux, marcy letoile, france, while excellent specificity was. Research tropheryma whipplei in children with gastroenteritis.
Sensitivity and specificity of the vikia r rotaadeno immunochromatographic test biomerieux and the elisa ideia tm rotavirus kit dako compared to genotyping. Performance analysis of two immunochromatographic assays. This is a qualitative test based on the immunochromatography technique. From january 2006 to july 2009, 467 samples of faeces from individuals with gastroenteritis. Update on schemes and the future uk neqas microbiology. Clostridium difficile infection in polish pediatric outpatients with inflammatory bowel disease d. Vikia rota adeno biomerieux diarlex mb fumouze b the two immunochomatographic tests were performed in the clinical laboratory of the debrousse hospital. Vikia biomerieux, kcombi set bioconnections, rida quick rbiopharm and quickstrip savyon diagnostics. Diagnostic accuracy of vikia rotaadeno and premier rotaclone tests for the. Interestingly, the specificity of the vikia rota adeno assay was 100%.
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